Table 2.
Plasmids used in this study
| Plasmid | Backbone | Genotype |
|---|---|---|
| pLG104 | pRS316 | PGAL1-10-3HA-BNR1(1-1473) |
| pLG106 | pRS316 | PGAL1-10-3HA-BNR1(1141-2199) |
| pLG130 | pRS316 | PGAL1-10-3HA-BNR1(1141-1473) |
| pLG164 | pRS316 | PGAL1-10-3HA-BNR1(1141-1818) |
| pLG186 | pRS316 | PGAL1-10-3HA-BNR1(1-1353) |
| pLG187 | pRS316 | PGAL1-10-3HA-BNR1(1-1368) |
| pLG188 | pRS316 | PGAL1-10-3HA-BNR1(1-1383) |
| pLG189 | pRS316 | PGAL1-10-3HA-BNR1(1-1398), 3HA-Bnr1-L1 |
| pLG190 | pRS316 | PGAL1-10-3HA-BNR1(1-1413) |
| pLG191 | pRS316 | PGAL1-10-3HA-BNR1(1-1428) |
| pLG192 | pRS316 | PGAL1-10-3HA-BNR1(1-1443) |
| pLG193 | pRS316 | PGAL1-10-3HA-BNR1(1-1458) |
| pLG194 | pRS316 | PGAL1-10-3HA-BNR1(1351-2199) |
| pLG195 | pRS316 | PGAL1-10-3HA-BNR1(1366-2199) |
| pLG196 | pRS316 | PGAL1-10-3HA-BNR1(1381-2199) |
| pLG197 | pRS316 | PGAL1-10-3HA-BNR1(1396-2199), 3HA-Bnr1-L2 |
| pLG198 | pRS316 | PGAL1-10-3HA-BNR1(1411-2199) |
| pLG199 | pRS316 | PGAL1-10-3HA-BNR1(1426-2199) |
| pLG200 | pRS316 | PGAL1-10-3HA-BNR1(1441-2199) |
| pLG201 | pRS316 | PGAL1-10-3HA-BNR1(1456-2199) |
| pLG202 | pRS316 | PGAL1-10-3HA-BNR1(1471-2199) |
| pLG311 | pRS316 | PBNR1-3GFP-BNR1(1-2199), 3GFP-Bnr1-L1-L2 |
| pLG314 | pRS316 | PBNR1-3GFP-BNR1(1396-2199), 3GFP-Bnr1-L2 |
| pLG320 | pRS316 | PGAL1-10-3GFP-BNR1(1-4128), 3GFP-Bnr1 |
| pLG325 | pRS316 | PGAL1-10-GFP-BNR1(1-1398), GFP-Bnr1-L1 |
| pLG328 | pRS316 | PGAL1-10-GFP-BNR1(1396-2199), GFP-Bnr1-L2 |
| pLG341 | pRS315 | PGAL1-10-mCherry-BNR1(1-1398), mCherry-Bnr1-L1 |
| pLG345 | pRS316 | PBNR1-3GFP-BNR1(1396-4128), 3GFP-Bnr1ΔL1 |
| pLG354 | pRS313 | PBNR1-3GFP-BNR1(1396-4128), 3GFP-Bnr1ΔL1 |
| pLG361 | pRS316 | PBNR1-3GFP-BNR1(1-1398, 2200-4128), 3GFP-Bnr1ΔL2 |
| pLG367 | pRS313 | PBNR1-3GFP-BNR1(1-1398, 2200-4128), 3GFP-Bnr1ΔL2 |
All plasmids were generated in this study.