Figure 2.

A Spspo13⁺-ScSec2 chimera rescues the growth defect of a sec2Δ. Strain HJ79 (sec2Δ; pCEN-URA3-ScSEC2) was transformed with CEN-TRP1-plasmids carrying the indicated genes. Numbers indicate amino acids. The transformed strains were grown in nonselective media overnight, and 10-fold serial dilutions of the overnight culture were spotted onto a synthetic plate lacking tryptophan and uracil or onto a plate containing 5-fluoroorotic acid (5-FOA). The plates were photographed after 2-d incubation at 30°C.