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Loss of GEF activity in SpSpo13 leads to an FSM assembly defect in vivo. Spspo13F79A cells do not form FSMs. Strain HJP1 (h90, spo13-B82) was cotransformed with plasmids carrying GFP-Psy1 and Spspo13+-mRFP or Spspo13F79A-mRFP, sporulated, and examined by fluorescence microscopy. Arrows indicate FSMs.
