Table 2.
Plasmids used in this study
| Plasmid | Selected features | Source |
|---|---|---|
| pRS416TEF | CEN, ARSH4, URA3 | Mumberg et al. (1995) |
| pRS414TEF | CEN, ARSH4, TRP1 | Mumberg et al. (1995) |
| pRS416TEF-ScSEC2 | CEN, ARSH4, URA3 | This study |
| pRS414TEF-ScSEC2 | CEN, ARSH4, TRP1 | This study |
| pRS414TEF-ScSEC21-160 | CEN, ARSH4, TRP1 | This study |
| pRS414TEF-ScSEC2161-759 | CEN, ARSH4, TRP1 | This study |
| pRS414TEF-Spspo13+ | CEN, ARSH4, TRP1 | This study |
| pRS414TEF-Spspo13+-ScSEC2161-759 | CEN, ARSH4, TRP1 | This study |
| pRS425TEF-Spypt2+ | 2 micron, LEU2 | This study |
| pGP564-ScSEC4 | 2 micron, LEU2 | Jones et al. (2008) |
| pGP564-ScYPT1 | 2 micron, LEU2 | Jones et al. (2008) |
| pGP564-ScYPT31 | 2 micron, LEU2 | Jones et al. (2008) |
| pJRU-MCS2 | YRp, ars1, ura4+ | Moreno et al. (2000) |
| pJRU-MCS2-PSpSpo13-Spspo13+ | YRp, ars1, ura4+ | This study |
| pJRU-MCS2-PSpSpo13-Spspo13-F79A | YRp, ars1, ura4+ | This study |
| pJRU-MCS2-PSpSpo13-Spspo13+-mRFP | YRp, ars1, ura4+ | This study |
| pJRU-MCS2-PSpSpo13-Spspo13-F79A -mRFP | YRp, ars1, ura4+ | This study |
| FY532a (pREP41-GFP-psy1+) | YRp, ars1, LEU2 | Nakamura et al. (2001) |
| pET15b | His-tag, AmpR | Novagen |
| pET15b-ScSEC4 | His-tag, AmpR | This study |
| pET15b-ScYPT1 | His-tag, AmpR | This study |
| pET15b-Spypt1+ | His-tag, AmpR | This study |
| pET15b-Spypt2+ | His-tag, AmpR | This study |
| pET15b-Spypt3+ | His-tag, AmpR | This study |
| pGEX5X-1 | GST, AmpR | GE Healthcare |
| pGEX5X-1-ScSEC21-160 | GST, AmpR | This study |
| pGEX3X-Spspo13+ | GST, AmpR | This study |
| pGEX3X-Spspo13-F79A | GST, AmpR | This study |
a This plasmid was obtained from the Yeast Genetic Resource Center of Japan supported by the National Bioresource Project (YGRC/NBRP) (http://yeast.lab.nig.ac.jp/nig/).