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Effect of altered NHE6.1 expression on endosomal pH. Serum-starved cells that had been treated with siRNA against NHE6.1 or overexpressed wild-type or mutant NHE6.1-mCherry were incubated with a combination of pH-sensitive fluorescein-labeled transferrin and pH-stable Alexa Fluor546- or Alexa Fluor633-labeled transferrin to measure endosomal pH as described in Materials and Methods. Data are presented as mean ± SD.
