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. Author manuscript; available in PMC: 2011 Mar 26.
Published in final edited form as: Mol Cell. 2010 Mar 26;37(6):865–878. doi: 10.1016/j.molcel.2010.01.039

Figure 1. Identification of MHF1 and MHF2 as integral components of the FA core complex.

Figure 1

(A) Immunoblotting showing Superose 6 gel-filtration profiles of FANCM, MHF1 and MHF2 in HeLa nuclear extract. The peak fractions and those corresponding to marker proteins are indicated at the bottom. (B) A silver-stained gel showing that the complex purified by a FANCM antibody from the pooled peak 1 fractions in (A) contained MHF1, MHF2 and other components of FA core and BLM complexes. IP indicates immunoprecipitation. (C) Immunoblotting shows that MHF1, MHF2 and other FA core complex components were present in the immunoprecipitates isolated from peak 1 fractions by MHF1, FANCM or FANCA antibodies. Nuclear extract (NE) was used as a loading control. (D) As described in (B), except a MHF1 antibody was used in IP. (E) Immunoblotting shows that MHF2 co-immunoprecipitated with MHF1 and other FA core complex components from HeLa cells stably expressing Flag-tagged MHF2, but not from control HeLa cells. A Flag antibody was used in IP. (F) As described in (B), except that peak 2 fractions in (A) were used for IP by a MHF1 antibody. (see also Figures S1 and S2).