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. 2010 Mar 31;5(3):e9924. doi: 10.1371/journal.pone.0009924

Figure 2. Multiparametric screen of known mitochondria-targeting molecules.

Figure 2

A. Mitochondria isolated from mouse liver, human non-cancerous (HME-1) and cancerous (PC-3) cells were treated with increasing concentrations of t-Bid, Bak BH3, HA-14.1, YC-137, Chelerythrine, Gossypol, TW-37, EM20-25 and ABT-737 before evaluation of mitochondrial swelling and ΔΨm loss. Alternatively, mitochondrial supernatant was subjected to ELISA assays for quantification of cytochrome c release. Effective concentration inducing 50% of the maximal effect (EC50) is given for swelling (100% of effect with 50 µM Ca2+), ΔΨm loss (100% of effect with 50 µM mClCCP) and cytochrome c release (100% of effect with 20 µg/ml alamethicin) (n = 3 independent experiments). B. Mitochondria isolated from mouse liver or HME-1, PC-3, HCT-116, HT-29 and Jurkat cell lines were incubated for 45 min at 30°C with increasing concentrations of ABT-737 and the supernatants were subjected to cytochrome c immunoblot (NT: untreated; Ala: alamethicin 20 µg/ml).