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. 2010 Mar 31;5(3):e9924. doi: 10.1371/journal.pone.0009924

Figure 3. ABT-737 induces relatively large MOMP in cancer cell mitochondria.

Figure 3

Isolated mitochondria from mouse liver, PC-3 and Jurkat cells were untreated (NT) or incubated either with alamethicin (Ala; 20 µg/ml; positive control), Bak BH3 peptide (10 µM), ABT-737 (1 µM) or recombinant t-Bid (1 nM) for 45 min. Mitochondrial supernatants were subjected to immunodetection of cytochrome c, Smac/DIABLO, Omi/Htra2 and AIF (Western blots are representative of 3 independent experiments). Note that cytochrome c (15 kDa), Smac/DIABLO (23 kDa), and Omi/Htra2 (37 kDa) but not AIF (56 kDa) are released from cancer cell mitochondria.