Fig. 1.
(A) DNA quantification analysis by PicoGreen™ DNA assay showed cells proliferating over time in different experimental groups. (B) Quantitative analysis of Ki67+ staining MCF10A cells counted in different microscopic fields. An inhibitory effect of stromal cells on epithelial cell growth was observed in the tri-cultures and co-culture during the first week. A significant difference between tri-culture and co-cultures was observed (*p < 0.05), while no difference exists between the two co-culture groups (#p > 0.05). The percentage of positive staining cells in each group decreased over time.
