Fig. 2.
Growth profile and viability of MCF10A cells in different cultures developed on silk scaffolds (at day 6). CLSM images showed both alveolar and duct-like structures formed in co-cultures (Top row, a1-c1), while only alveolar structures were observed in the monocultures (d1). (Red, MCF10A cells labeled with Di I™; Green, stromal cells labeled with CMFDA). Viability of MCF10A cells in different groups detected by viability staining (middle row, a2-d2)). H&E staining showed morphological characteristics of the epithelial structures formed by MCF10A cells in different groups (bottom row, a3-d3). (Arrowhead notes duct-like structure, asterisk notes alveolar structure).
