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Quantitative analysis of the percentage of duct-like epithelial structures generated by MCF10A cells in the different groups. Compared with the co-cultures, the highest percentage of ductal structures (Nduct/N(acini +duct) ×100%) was found in the tri-culture (*p < 0.05). No significant difference exists between the two co-culture groups (#p > 0.05). The percentage of ductal structures in each group increased over time.
