Fig. 5.
Immunostaining with sialomucin (red) and GM130 (green) showed the polarity of the alveolar structures formed by tri-cultured MCF10A cells on silk scaffold (b1-b3, day 6), which is similar to the positive control of human native breast tissue (a1-a3) in comparison to the co-cultures (c1-c3, d1-d3). The cell nucleus was counter-stained by DAPI (blue). Reversed polarity was also observed in the monoculture (e1-e3, day 6).
