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Influence of BTC upon osteoclast regulatory factors. hMSC were cultured under osteogenic conditions in the presence of 0.5–500 ng/ml BTC for 7–21 days, from which total RNA lysates were examined by qtPCR analysis for (a) IL-6, (b) RANKL, (c) OPG, or (d) RANKL/OPG. Bars Means ± SE. *P<0.05 compared with BTC-free culture within same timepoint. n=3–4 per experiment per time point
