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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Immunol. 2009 Oct 14;183(9):5468–5476. doi: 10.4049/jimmunol.0902344

Figure 5. Pre-pro to pro-B block in Mb1-Cre ShcFFF mice and Shc tyrosine phosphorylation in response to IL-7 occur independently of the pre-BCR.

Figure 5

(A) CD19+ cells from Rag1-/- bone marrow were allowed to rest for 1 hour in plain RPMI before incubation with either no IL-7 or 100ng/mL IL-7 for the indicated times. Total lysates were probed with antibodies against phospho-Shc (pY239/pY240) and then total Shc. (B) Total bone marrow cell numbers. Block in pre-pro-B to early pro-B in Mb1-Cre ShcFFF mice on a Rag1-/- background. (C) Pro-B cells from Mb1-Cre ShcFFF Rag1-/- mice express IL-7 receptor at levels comparable to littermate controls. (D) CD19+ bone marrow pro-B cells from mice on a Rag1-/- background were grown in varying concentrations of IL-7, and proliferation was measured after four days. Cell growth was assessed with [3]H incorporation (left panel) or by total cell counts (right panel). (E) CD19+ pro-B cells from mice on a Rag1-/- background were grown on OP-9 stromal cells in the presence of 5ng/mL IL-7 for the indicated times. Total cell counts were quantified with a flow cytometry-based bead assay.