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Overexpression of Sec3p leads to an accumulation of Sec61p-GFP but not Hmg1p-GFP at the bud tip. Representative images of Sec61p-GFP (A and B) and Hmg1p-GFP (C and D) in a Sec3p overproducer, NY2463 (sec3::kanMX, pRS426-SEC3; A and C) and in a control strain, NY1211 (SEC3; B and D) are shown. Arrows point to either small buds or medium-sized buds.
