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. 2010 Mar 31;2(2):e00032. doi: 10.1042/AN20100003

Figure 8. Complex 2 is formed by DNA–protein interactions involving nucleotides upstream of the polymorphic site.

Figure 8

(A) Shown is the 30 bp sequence delineating unlabelled competitor DNAs for the 129B6 polymorphic region. The polymorphic site is denoted by an asterisk. Fragments a, b and c span the individual regions shown. These fragments were used for competition as concatemers of three copies of each 10 bp region. (B) Representative PhosphorImager-generated autoradiograph of unlabelled co-competition experiments with different competitor DNAs reacting with 129B6 retinal extract. The radiolabelled 129B6 full-length probe was incubated in the presence or absence of unlabelled competitor DNAs. Each competitor DNA was used at a 150-fold molar excess of potential binding sites. Competitor a specifically competed Complex 2, while b competed Complex 1, but minimally competed Complex 2. Alternatively, oligo bc efficiently competed Complex 2. These results suggested Complex 2 was formed with interactions upstream of the polymorphic site, but may also involve interactions further downstream of this site.