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. Author manuscript; available in PMC: 2011 Apr 6.
Published in final edited form as: Biochemistry. 2010 Apr 6;49(13):2962–2972. doi: 10.1021/bi902218p

Figure 2.

Figure 2

Recombinant LOX-PP contains both O- and N- linked sugars. Recombinant LOX-PP was treated with a combination of deglycosidases to remove N- and/or O-linked sugars followed by resolution on a reducing SDS PAGE gel and stained with Coomassie G-250 based SimplyBlue™ SafeStain. Lane 1: rLOX-PP, lane 2: rLOX-PP + PNGase F, lane 3: rLOX-PP + PNGase F + O-glycosidases (Neuraminidase and O-glycosidase), lane 4 rLOX-PP + O-glycosidases only (Neuraminidase and O-glycosidase), lane 5: PNGase F alone. Molecular weight markers are indicated in kDa. All shifted bands are immunoreactive with the anti-LOX-PP antibody on a Western blot (Figure S1).