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. Author manuscript; available in PMC: 2010 Mar 31.
Published in final edited form as: Laryngoscope. 2007 Jan;117(1):86–91. doi: 10.1097/01.mlg.0000244182.81768.31

Fig. 2.

Fig. 2

Peptidoglycan-polysaccharides (PGPS) stimulates production of interleukin (IL)-8 in bronchial and middle ear epithelial cells. Luciferase assays indicated that PGPS increased transcription of IL-8 in NL-20 (A and B), rat middle ear epithelial cells (MEECs) (E), and mouse MEECs (F) in dose- and time-dependent manner. Enzyme-linked immunosorbent assay verified that PGPS at 0.5 μg/mL stimulated production of IL-8 peptide in NL-20 in dose- (C) and time-dependent (D) manner. It was noted that normal NL-20 cells produce very little IL-8 (D, phosphate buffered saline), although IL-8 mRNA transcription was detected (A and B). *P < .05.