Skip to main content
. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Arch Otolaryngol Head Neck Surg. 2009 Jul;135(7):694–703. doi: 10.1001/archoto.2009.78

Figure 3. LCM and 2D-DIGE.

Figure 3

Panels A-E illustrate the process of LCM. Panel A is the hematoxylin and eosin stained pilot section. B shows the nuclear fast red stained sample prior to LCM. In C, the black circles indicate areas where the laser has impacted the tissue. D shows the leftover tissue that remains on the slide. E shows the captured tissue. Panel F shows a representative merged image of a 2D gel. Proteins from the captured tissue (tumor labeled with Cy5 and shown in red, mixed internal standard labeled with Cy3 and shown in green) are separated based on isoelectric point and molecular weight. Proteins more abundant in the mixed internal standard are green, proteins more abundant in cancer are red and equally expressed proteins are yellow.