Table 2. List of yeast strains used and constructed in this study.

Strain number	Relevant genotype	Genotype	Reference
Y67	wt	his3Δ1, leu2Δ0, met15Δ0, ura3Δ0	BY4741–(EUROSCARF)
Y75	weak [PSI+][PIN+]	MATa, ade1–14, his3-Δ200, leu2.3,112, trp1-289, ura3-52, weak [PSI+][PIN+]	OT55 [31]
Y81	[psi−][pin−]	MATa, ade1–14, his3-Δ200 or 11,15, leu2.3,112, trp1-Δ, ura3-52, lys2, [psi-][pin−] dx. of GT81-1C (Y82) (GuHCl-cured)	GT409 [32]
Y103	mca1Δ	his3Δ1, leu2Δ0, met15Δ0, ura3Δ1, YOR197w::kanMX4	BY4741–(EUROSCARF)
Y119		ade1–14/ade1–14, his3-Δ200 (or 11,15)/his3-Δ200, (or 11,15), leu2.3,112/leu2.3,112, trp1-Δ/trp1-Δ, ura3-52,ura3-52, lys2/lys2	GT81 [25]
Y133	SUP35+/sup35Δ [PSI+]	ade1-14/ade1–14, his3-Δ200 (or 11,15)/his3-Δ200 (or 11,15), leu2.3,112/leu2.3,112, trp1-Δ/trp1-Δ, ura3-52,ura3-52, lys2/lys2 SUP35+/sup35Δkanmx6 [PSI+]	this study
Y312	Δsup35/V236	MATa, ade1–14, his3-Δ200 (or 11,15) leu2.3,112, trp1-Δ, ura3-52, lys2, sup35::kanmx; Δsup35/V236 (p2HG-SUP35C)	this study
Y316	Δsup35/V124	MATα, ade1–14, his3-Δ200 (or 11,15), leu2.3,112, trp1-Δ, ura3-52, lys2, sup35::kanmx; Δsup35/V124 (p2HG-MCA1N-SUP35C)	this study
Y320	Δsup35/V123	MATα, ade1–14, his3-Δ200(or 11,15), leu2.3,112, trp1-Δ, ura3-52, lys2, sup35::kanmx; Δsup35/V123 (p2HG-MCA1-SUP35C)	this study
Y322	Δsup35/V257	MATa, ade1–14, his3-Δ200(or 11,15), leu2.3,112, trp1-Δ, ura3-52 lys2 sup35::kanmx, Δsup35/V257 (p2HG-MCA1C-SUP35C)	this study

Details for construction of Y133 and primer sequences are given in Materials and Methods.