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. 2010 Apr;135(4):333–353. doi: 10.1085/jgp.200910336

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© 2010 Toulme et al.

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Figure 6. — Properties of ATP-evoked currents in activated C8-B4 cells. (A; left) Concatenated traces of membrane voltage ramps applied before, during, and after the application of 100 µM ATP to a single activated C8-B4 cell. The traces labeled as a, b, and c are shown expanded on the current–voltage relation on the right. The current–voltage relation of the ATP-evoked current is shown in blue for this representative cell. For this, we used b–c to calculate I_ATP. (B) The average data for five resting and five activated C8-B4 cells. For activated C8-B4 cells, the ATP-evoked current was slightly inwardly rectifying and reversed direction at +5 mV. ATP failed to evoke any inward currents in resting C8-B4 cells. (C) The black traces are ATP-evoked currents (ampere units), the red traces are the integral of the current (Q_T in nC units), and the blue traces are the ΔF₃₈₀ of FURA (in BUs). ATP evoked negligible inward currents and changes in FURA fluorescence in resting C8-B4 cells (−LPS), but it evoked significant inward currents and changes in FURA fluorescence in activated C8-B4 cells. Traces such as these were used to calculate the Pf% of the ATP-evoked currents in activated C8-B4 cells, as described in Materials and methods. (D) Current–voltage relations for 100-µM ATP-evoked currents in activated C8-B4 cells in the presence of NMDG⁺ as major extracellular cation. (E) Plots YOPRO1 fluorescence intensity over time for five and six resting and activated C8-B4 cells. 100 µM ATP failed to evoke YOPRO1 uptake in resting or activated C8-B4 cells, whereas cell permeabilization with 0.1% Triton did so. All of the ATP-evoked currents were measured at a holding voltage of −60 mV.