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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Blood. 2009 Jun 24;114(9):1794–1802. doi: 10.1182/blood-2009-04-216770

Figure 3. TLR7 triggers expression of IFN-inducible genes in a type I IFN-independent manner.

Figure 3

GEN2.2 cells were untreated or stimulated with Flu or CL097 for different times. Supernatants were collected for evaluation of (A) IFN-α and (B) CXCL10 production. (C) RNA was extracted for measurement of MxA transcription levels by real-time PCR. (D) TRAIL expression was evaluated by flow cytometry. GEN2.2 cells were untreated or stimulated with Flu or CL097 in the absence or presence of anti-IFN-α/β and anti-IFN-α/βR neutralizing antibodies for 4 h. (E) TRAIL expression was evaluated by flow cytometry and (F) CXCL10 production was measured in culture supernatants by CBA. Data shown are the means and standard deviation from duplicate values from two independent experiments except data in (C) which are representative of two independent experiments.