Table 3. Metaphyseal bone structural parameters determined by in vivo μCT, ex vivo specimen μCT, and bone histomorphometry 10 weeks after tumor inoculation.

Technique and parameter	Model	Threshold	Mean Value
			Femur w/o tumor	Femur with tumor	P
In vivo μCT, 91 µm
TbN (/mm)	O*	400	2.9	1.6	<0.05
	A*	633±220	2.4	2.2	<0.50
TbSp (mm)	O	400	0.11	0.26	<0.002
	A	633±220	0.41	0.54	<0.50
BV/TV (%)	O	400	0.89	0.4	<0.002
	A	633±220	0.25	0.16	<0.08
TbTh (mm)	O	400	0.3	0.13	<0.001
	A	633±220	0.13	0.11	<0.13
BMD (mg/cm3)	O	400	217	150	<0.004
	A	633±220	227	150	<0.004
Ex vivo μCT, 31 µm
TbN (/mm)	O	2000	2.88	1.2	<0.005
	A	1830±650	2.95	2.24	<0.12
TbSp (mm)	O	2000	0.5	9.96	<0.05
	A	1830±650	0.32	0.45	<0.48
BV/TV (%)	O	2000	0.11	0.05	<0.02
	A	1830±650	0.12	0.11	<0.78
TbTh (mm)	O	2000	0.039	0.034	<0.15
	A	1830±650	0.041	0.054	<0.03
BMD (mg/cm3)	O	2000	451	342	<0.02
	A	1830±650	463	342	<0.02
Ex vivo μCT, 16 µm
TbN (/mm)	O	2000	4.2	1.77	<0.001
	A	2423±770	3.4	2.14	<0.03
TbSp (mm)	O	2000	0.29	1.08	<0.02
	A	2423±770	0.3	0.54	<0.20
BV/TV (%)	O	2000	0.13	0.05	<0.005
	A	2423±770	0.1	0.06	<0.03
TbTh (mm)	O	2000	0.032	0.035	<0.56
	A	2423±770	0.03	0.034	<0.20
BMD (mg/cm3)	O	2000	475	344	<0.006
	A	2423±770	482	353	<0.006
Ex vivo μCT, 8 µm
TbN (/mm)	O	3000	5.7	2.4	<0.001
	A	1822±920	13	37	<0.001
TbSp (mm)	O	3000	0.25	2.17	<0.05
	A	1822±920	0.1	0.05	<0.06
BV/TV (%)	O	3000	0.1	0.04	<0.02
	A	1822±920	0.16	0.31	<0.03
Tb Th (mm)	O	3000	0.02	0.016	<0.40
	A	1822±920	0.015	0.013	<0.39
BMD (mg/cm3)	O	3000	500	333	<0.006
	A	1822±920	486	339	<0.006
Histomorphometry
TbN (/mm)			3.96	2.45	<0.003
TbSp (mm)			0.22	0.29	<0.05
BV/TV (%)			10	6	<0.006
TbTh (mm)			0.036	0.039	<0.27

*O, optimal threshold; A, automated threshold.