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. 2010 Mar 26;6(3):e1000829. doi: 10.1371/journal.ppat.1000829

Figure 6. Human fibroblasts express SR-As that mediate dsRNA binding via the collagenous domain.

Figure 6

(A) SR-A transcripts were detected in primary human fibroblasts (Hel) using RT-PCR and primers specific to SR-AI (lane I), SR-AII (II), MARCO (M), SCARA3 variant 1(3v1), SCARA3 variant 2 (3v2), SCARA4 (4), and SCARA5 (5). SR-A transcripts detected from immortalized human embryonic kidney cells (293) and primary human peripheral blood mononuclear cells (hPBMCs) were included for comparison purposes. (B) SR-A expression at the protein level was measured in human fibroblasts by western blot analysis using a whole cell extract (WCE). A single band ∼70kDa in size was detected. (C) Alexafluor 488 labelled v1000 (1 µg/mL) binding was observed in HEL cells by fluorescence microscopy with or without pre-treatment with the SR-A competitive ligand fucoidin or fetuin, its non-competitive counterpart (both at 100 µg/mL). Also, v1000 binding was monitored in the presence of the anti-human SRA antibody (αSRA) and a normal goat serum (ngs) control (both diluted at 1∶50).