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PfuX7 accepts dUTP in place of dTTP in PCR. Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard dNTPs or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
