Figure 9.

Genetic interactions within and between the Spc105p, Mtw1p, Ndc80p, and Dam1p/DDD/DASH complexes. (A) Suppression of spc105-4 by overexpression of Ydr532p. Strains were MSY150, MSY151, and MSY152. (B) Examples of the synthetic growth defects at 30°C between mutations in the different complexes. (C) A summary of the interactions found. Solid lines show synthetic-lethal interactions at 23°C, dashed lines show synthetic growth defects with lethality at 30°C (see panel B), the dotted line shows dosage suppression (see panel A), and the absence of a line indicates that no genetic interaction was found. All possible combinations between the different mutations were tested; however, the YDR532c dosage suppression was only tested in the spc105 alleles. The synthetic-lethal interactions within the Ndc80p and Mtw1p complexes have been described previously (Wigge and Kilmartin, 2001; Euskirchen, 2002). The ask1, dam1, and dad1 alleles are not distinguished because they gave similar results in all the crosses tested, with the exception of the dam1-31 mtw1-11 cross, in which no interaction was found. duo1 is excluded from the ask1, dad1, and dam1 group because duo1-61 showed no genetic interactions with any of the other alleles. Strains used were JK1121, JK1504, MSY72, MSY79, MSY87, MSY90, PWY483, PWY754, VNY69, VNY87, VNY96, and VNY162.