Table 2.
Plasmid list
| Plasmid | Description | Source |
|---|---|---|
| pRS306 | URA3 (integrating) | Sikorski and Heiter (1989) |
| pRS314 | CEN TRP1 (low copy) | Sikorski and Heiter (1989) |
| pRS316 | CEN URA3 (low copy) | Sikorski and Heiter (1989) |
| pRS426 | 2μ URA3 (high copy) | Christianson et al. (1992) |
| pJK472 | GFP5-Sphis5+ in pBluescript SK- | Wigge et al. (1998) |
| pJK474 | pA-Sphis5+ in pBluescript SK- | Wigge and Kilmartin (2001) |
| pJK544 | GFP5-kanMX4 in pBluescript SK- | This article |
| pJK578 | SPC42-CFP in pRS304 | This article |
| pJK19.9-4 | spc105-4 in pRS306 | This article |
| pJK19.9-15 | spc105-15 in pRS306 | This article |
| pJK19.11-68 | YDR532c in pRS426 | This article |
| pJK4-7a | mtw1-11 in pRS314 | This article |
| pMS27.9 3-1 | ask1-22 in pRS306 | This article |
| pMS27.9 6-1 | duo1-61 in pRS306 | This article |
| pMS2.10 3-1 | dad1-13 in pRS306 | This article |
| pMS18.9 3-1 | dam1-31 in pRS306 | This article |
| pPAW1(3.10 1-1) | SPC105 in pRS316 | Wigge and Kilmartin (2001) |
| pVN7.10 1-1 | 3HA-KITRP1 in pBluescript SK- | This article |
| pVN10.5 137 | nsl1-6 in pRS314 | This article |
| pVN11.4 77-2 | nnf1-77 in pRS314 | This article |
| pVN17.5 157 | nsl1-5 in pRS314 | This article |
| pVN28.5 186-2 | dsn1-8 in pRS306 | This article |
| pVN28.5 187-3 | dsn1-7 in pRS306 | This article |
Sphis5+ is the S. pombe his5+ module from pFA6a-His3MX6 and kanMX4 the kanamycin resistance module from pFA6a-kanMX4 (Wach et al., 1997); KlTrp1 is the Kluyveromyces lactis TRP1 gene (Stark and Milner, 1989).