Figure 4.
Mapping of aldolase-binding site on TRAP. (A) Peptides derived from the P. falciparum TRAP C-terminus sequence were tested for their ability to interfere with the binding between biotin-labeled PfAldo and the TRAP34mer peptide as indicated in legend to Figure 3. The sequence, length, and concentration required to obtain 50% inhibition (IC50%) are shown. (B) Equal amounts of each GST fusion protein were tested for their ability to interact with PfAldo by pull-down assays as described in Figure 2B.