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. 2003 Dec;14(12):4958–4970. doi: 10.1091/mbc.E03-06-0426

Figure 2.

Figure 2.

A multicopy RSR1 plasmid, but not plasmids carrying other rsr1 alleles, suppressed the growth defect of cdc42-118. (A) Dilution series compared growth at 25 and 36°C (SC-URA, 4d) of wild-type and mutant yeast transformed with a high-copy (YEplac195) 2-μm plasmid containing RSR1 or no insert. Each spot of cells represented a 10-fold serial dilution from left to right. From top to bottom, RSR1, 2 μm-plasmid containing strains are KKY 66, 68, 287, 289, 291, and 190; strains containing only 2 μm-plasmid are KKY 239, 240, 286, 288, 290, and 238. (B) Wild-type and cdc42-118 strains were transformed with 2-μm plasmids (shown in brackets) containing no insert, a genomic fragment containing RSR1, alleles of RSR1, or CDC24 and grown at 25 (top panel) or 37°C (bottom panel) for 3 d on SC-URA. The strains shown (clockwise from 1 o'clock) are KKY 238, 240, 1012, 68, 60, 201, 200, and 69. Note: RSR1 suppressed cdc24-118 better than CDC24. (C) The cdc42-118 and cdc24-4 mutants, transformed with 2-μm plasmids containing RSR1, rsr1-7, or no insert, were grown at 25°C (for 3 d) or 37°C (for 4 d) on SC-URA (for cdc42-118) or SC-URA + 1 M sorbitol (for cdc24-4). Tenfold serial dilutions of each cdc42-118 transformant and 2.5-fold serial dilutions of each cdc24-4 transformant are shown.