Table 1.
S. cerevisiae strains used in this study
| Strain | Relevant genotype | Source |
|---|---|---|
| DDY1102 | MATa/MATα his3Δ200/his3Δ200 leu2-3,112/leu2-3,112 ura3-52/ura3-52 ADE2/ade2-1 lys2-801am/LYS2 | Kozminski et al. (2000) |
| DDY1300 | CDC42:LEU2 | Kozminski et al. (2000) |
| DDY1304 | cdc42-101:LEU2 | Kozminski et al. (2000) |
| DDY1326 | cdc42-118:LEU2 | Kozminski et al. (2000) |
| DDY1336 | cdc42-123:LEU2 | Kozminski et al. (2000) |
| DDY1344 | cdc42-129:LEU2 | Kozminski et al. (2000) |
| KKY37 | MATarho1-104 leu2-3,112 ura3-52 lys2-801am | This study |
| KKY238 | CDC42:LEU2 [pKK1365, 2μ vector YEplac195] | This study |
| KKY239 | cdc42-101:LEU2 [pKK1365] | This study |
| KKY240 | cdc42-118:LEU2 [pKK1365] | This study |
| KKY286 | cdc42-123:LEU2 [pKK1365] | This study |
| KKY288 | cdc42-129:LEU2 [pKK1365] | This study |
| KKY290 | rho1-104 [pKK1365] | This study |
| KKY1010 | CDC42:LEU2 [pKK1369, 2μ YEp24 library clone] | This study |
| KKY1011 | cdc42-101:LEU2 [pKK1369] | This study |
| KKY1012 | cdc42-118:LEU2 [pKK1369] | This study |
| KKY1013 | CDC42:LEU2 [pKK980, 2μ YEp24 library clone rsr1Δ] | This study |
| KKY1014 | cdc42-118:LEU2 [pKK980] | This study |
| KKY190 | CDC42:LEU2 [pKK925, YEplac195 (RSR1)] | This study |
| KKY66 | cdc42-101:LEU2 [pKK925] | This study |
| KKY68 | cdc42-118:LEU2 [pKK925] | This study |
| KKY287 | cdc42-123:LEU2 [pKK925] | This study |
| KKY289 | cdc42-129:LEU2 [pKK925] | This study |
| KKY291 | rho1-104 [pKK925] | This study |
| KKY256 | CDC42:LEU2 [pKK1095, YEplac195 (rsr1T35A)] | This study |
| KKY60 | cdc42-118:LEU2 [pKK1095] | This study |
| KKY193 | CDC42:LEU2 [pKK1240, YEplac195 (rsr1G12V)] | This study |
| KKY200 | cdc42-118:LEU2 [pKK1240] | This study |
| KKY194 | CDC42:LEU2 [pKK1243, YEplac195 (rsr1K16N)] | This study |
| KKY201 | cdc42-118:LEU2 [pKK1243] | This study |
| KKY69 | cdc42-118:LEU2 [pHP569, YEp103 (CDC24)] | This study |
| Y147 | MATacdc24-4 ura3 leu2-3, 112 his3 | Bender and Pringle (1989) |
| KKY342 | CDC42:LEU2 rsr1::HIS3 | This study |
| KKY343 | cdc42-101:LEU2 rsr1::HIS3 | This study |
| KKY344 | cdc42-101:LEU2 RSR1 | This study |
| KKY345 | cdc42-118:LEU2 rsr1::HIS3 | This study |
| KKY346 | cdc42-118:LEU2 RSR1 | This study |
| KKY297 | cdc42-129:LEU2 rsr1::HIS3 | This study |
| KKY298 | cdc42-129:LEU2 RSR1 | This study |
| KKY85 | MATa/MATα rsr1::HIS3/RSR1 his3Δ200/his3Δ200 leu2-3,112/leu2-3,112 ura3-52/ura3-52 ADE2/ade2-1 lys2-801am/LYS2 | This study |
| KKY86 | MATα rsr1::HIS3 ura3-52 leu2-3,112 his3Δ200 lys2-801 am | This study |
| Y3611 | MATa/MATα can1Δ::MFA1pr-HIS3-MFα1pr-LEU2/CAN1 his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 met15Δ0/MET15 lys2Δ0/LYS2 | This study |
| Y3598 | MATacan1Δ::MFA1pr-HIS3-MFα1pr-LEU2 his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 | This study |
| Y2420 | MATamfa1Δ::MFA1pr-HIS3 ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 | Tong et al. (2001) |
| Y2823 | MATα mfα1Δ::MFα1pr-LEU2 his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | Tong et al. (2001) |
| BY4741 | MATahis3Δ1 leu2Δ0 ura3Δ0 met15Δ0 | Brachmann et al. (1998) |
| BY4742 | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | Brachmann et al. (1998) |
| KKY281 | MATα CDC42:natMX can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | This study |
| KKY283 | MATα cdc42-118:natMX can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | This study |
| YD00000-YD09999 | MATaorfΔ::kanMX4 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Winzeler et al. (1999) |
| HPY16 | MATaura3-52 his3-Δ1 leu2 trp1Δ63 prb1-1122 pep4-3 prc1-407 | Park et al. (1993) |
All cdc42:LEU2 and CDC42:LEU2 strains above are MATa ura3-52 leu2-3,112 his3Δ200 lys2-801am and are isogenic except at CDC42. The strain background is S288C. Ten back-crosses transferred rho1-104 (Yamochi et al., 1994) into the S288C DDY/KKY strain background. Plasmid bearing KKY strains were isolated as transformants of DDY1300, DDY1304, DDY1326, DDY1336, DDY1344 (Kozminski et al., 2000), and KKY37, respectively.