Figure 2. Sp1, AP-1 and c-Rel bind to their cognate motifs present within the hres regulatory sequences.

A. Sp1 binds specifically to the hres cognate motif. EMSA with Sp1 binding motif using U937 cells and supershift with Sp1, Sp3 and Sp4 antibodies. EMSA using nuclear extract prepared from U937 cells shows AP-1 binding to its hres cognate motif (B) and weak binding of c-Rel to its hres cognate motif (C). Arrows indicate the position of binding of the corresponding trans-acting factors to their cognate motif and the super-shifts using antibodies. These experiments were repeated two times.