Figure 7. Sp1 and PPARγ transcription factors interact as evident from co-immuno precipitation and western blot analyses.

Co-immuno precipitation with Sp1 and PPARγ was performed using cell extracts prepared from PMA stimulated (PMA) or un-stimulated (US) U937 cells. Immuno-precipitates were immuno-blotted with Sp1 and PPARγ antibodies. Bands corresponding to Sp1 and PPARγ are indicated by black arrows. The IgG heavy chain is indicated in blue. This experiment was repeated twice.