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Fibronectin is a substrate for plasmin bound to FT. FTLVS (109 CFU) were incubated with 100 μg of huPLG and 0.5 μg tissue tPA for 1 hour at 37°C. After removal of unbound huPLG and tPA, 3 μg fibronectin was added and allowed to incubate for 24 hours at 37°C. Supernatant from each preparation were separated by SDS-PAGE and transferred to PVDF membrane. Degradation of fibronectin was detected by Western blot analysis as described in "Materials and Methods".
