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. 2010 Mar 29;5(3):e9794. doi: 10.1371/journal.pone.0009794

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Chuykin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A): Rat blastocyst containing CFSE- labelled cells in the trophectodermal layer (arrow), merged image of carboxyfluorescein succinimidyl ester (CFSE) fluorescence and Hoechst 33342 is shown, arrowhead indicates non-included cells in blastocoel. (B): Rat blastocyst with B10 cell expressing EGFP in the trophectoderm layer (arrow), fluorescent image and merged image of EGFP with differential interference contrast (Dic) is shown. Photos were taken with 40× objective.