Table 1.

Properties of PH_Crac-GFP at leading edge of cells in a cAMP gradient and of PH_Crac-GFP in patches of cells in uniform cAMP

Property	PHCrac-GFP at leading edge in cAMP gradienta	PHCrac-GFP at patch in uniform cAMP
Size (μm)	7.1 ± 1.4	9.0 ± 3.2
Lifetime(s)	51 ± 6	53 ± 16
Number/cell	1	2.3 ± 1.1
Time between PHCrac-GFP and pseudopod extension(s)	5—15	12—16
Half-maximal inhibition by LY294002 (μM)	∼20b	∼15

Data shown are the means and SD deviations of at least six determinations.

^aData were obtained by analyzing movies from Parent et al. (1998) as in Figure 7

^bInhibition by LY294002 was derived from Funamoto et al. (2001)