Figure 3.

Phosphorylation (phos) of group IVa phospholipase A₂ (gIVaPLA₂) caused by IL-8/CXCL8 activation. a) Treated PMNs were activated with 100 ng/ml IL-8/CXCL8 at different time intervals, in minutes (min) and were lysed immediately. Treated samples were subjected to Western blot analysis and probed with pAb directed against Ser⁵⁰⁵ gIVaPLA₂ (top) or pAb against total gIVaPLA₂ (bottom). Total gIVaPLA₂ protein was stained with pAb directed against total gIVaPLA₂ to indicate equal loading of samples. Representative immunoblot from 3 independent experiments. b) Translocation of cytosolic gIVaPLA₂ to the nuclear component of PMNs was examined by subfractionation. Representative blot from 3 different donors was shown after staining with mAb against gIVaPLA₂.