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AMPK promotes Akt phosphorylation independent of IRS1. A. 3T3-F44a preadipocytes were treated with AICAR (1 mM, 2 h), followed by EGF (10 ng/ml, 15 min) and the cell extracts blotted with antibodies, as indicated. B. 3T3-F44a preadipocytes containing DNα1 or the empty viral vector were incubated with AICAR (1 mM) for different time, and the cell extracts blotted with antibodies, as indicated.
