Northern blot analyses show ZBED4 mRNA in various human tissues and Y79 cells. (A) A human multitissue blot, and a separate blot containing human retina and Y79 cells mRNAs were probed with a 32P-labeled ZBED4 cDNA. Two transcripts were detected when using a 3′ UTR probe (HZB22). (B) An additional 4-kb transcript was detected in retina and Y79 cells when using a 5′ UTR probe (HZB4). Lanes: 1, brain; 2, colon; 3, heart; 4, kidney; 5, liver; 6, lung; 7, muscle; 8, placenta; 9, small intestine; 10, spleen; 11, stomach; 12, testis; 13, retina; and 14, Y79 cells.