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. Author manuscript; available in PMC: 2010 Mar 31.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2009 Apr 15;50(8):3580–3588. doi: 10.1167/iovs.08-2751

Table 1.

PCR Primers

Primer Forward Reverse Fragment Size (bp)
HZB4 5′-CAGCCGGAGTAGTTATGGTCA-3′ 5′-GCAGACACACTGCCATTTTC-3′ 557
HZB22 5′-GCGTTAGCAGCCTGTACCA-3′ 5′-AATGCCACGTCTCTGTCGTA-3′ 532
KpnI-ZB-SmaI 5′-TTGGTACCTATGGAGGATAAGCAAGAAAC-3′ 5′-ACATCTTCCCGGGATACATC-3′ 1232
SmaI-ZB-HindIII 5′-TATCCCGGGAAGATGTGTCCA-3′ 5′-GAAGCTTTGTAGCGAGGGTC-3′ 1825
Pf1M1-ZB-NotI 5′-CAAGAGCCAGAGGATGGTGCAGAACTTAC-3′ 5′-TGGCGGCCGCTCATCAATACTGAAAGCATATTAAAG-3′ 985
ZB-38 (ZBED4) 5′-TCGTGGCCTGGGCTGTCTACCTTA-3′ 5′-GGGCTTCTGAAAACGCACCTTGAC-3′ 382
ZB-27 (ZBED4) 5′-GCTCACTGGGTCACCTTCGCATCC-3′ 5′-CCAGGCTTCCCACCAACACTCCAG-3′ 144
mA1 (mouse β-actin) 5′-GACATCCGTAAAGACCTCTAT-3′ 5′-TTGATCTTCATGGTGCTAGGA-3′ 122
mA2 (mouse β-actin) 5′-CCTAAGGCCAACCGTGAAAAGATG-3′ 5′-ACCGCTCGTTGCCAATAGTGATG-3′ 430
PDEα 5′-ATCTTGGAAAGGCATCATTTGG-3′ 5′-GCGATTCAGGTTCTGGAAGATATT-3′ 81
PDEα′-1 5′-AAGAGAACCATGTTTCAAAAGATC-3′ 5′-GGAGGTTACATATTTGATGGTTTCTTC-3′ 81
PDEα′-2 5′-CTGGGGGTGATCTTCAAAAAG-3′ 5′-GTTCACTGCCATGACCACAG-3′ 498