Table 2.

Effects of hRap1 mutants on telomere length heterogeneity

hRap1 allele	PDa	Median lengthb	50% signal intervalc	Heterogeneity indexd
hRap1	0	1.9	1.7	0.89
hRap1ΔCT	0	3.2	3.0	1.25
hRap1ΔBr	0	2.8	2.6	0.93
hRap1ΔBrΔCT	0	2.9	3.2	1.09
hRap1ΔMΔC	0	2.6	2.9	1.10
pLPC	108	2.6	3.3	1.25
pLPC	120	2.6	2.9	1.13
hRap1	120	4.8	5.2	1.10
hRap1ΔCT	108	7.7	6.9	0.90
hRap1ΔCT	120	7.8	6.7	0.86
hRap1ΔBr	120	9.0	4.8	0.53
hRap1ΔBr	132	9.1	5.0	0.55
hRap1ΔBrΔM	108	9.7	4.1	0.47
hRap1ΔBrΔM	180	11.8	5.6	0.47
hRap1ΔMΔC	96	10.0	7.5	0.75
hRap1ΔMΔC	108	10.9	7.3	0.67
hRap1ΔM	132	11.6	9.0	0.78

^aPD 0 represents the first split after selection for retroviral infection. The number of population doublings at this PD could vary slightly for different hRap1 mutants

^bMedian telomere length (in kb) was determined based on telomeric restriction fragment blots by scanning through the lanes. The portion of subtelomeric DNA on telomeric restriction fragments is assumed to be 2 kb, and this length is subtracted from all values

^c50% signal interval (in kb) represents the size distribution covering 50% of the telomeric signal (MATERIALS AND METHODS)

^dThe heterogeneity index represents the ratio of the 50% signal interval (fourth column) and the median telomere length (third column)