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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Dev Dyn. 2009 Jul;238(7):1727–1743. doi: 10.1002/dvdy.21994

Fig. 1.

Fig. 1

Injection-mediated Sleeping Beauty transgenesis in Xenopus. a: Schematic representation of the injection method used to generate the SB transgenic frogs. Xenopus laevis embryos were injected at the one-cell stage with pTβGFP plasmid and mRNA encoding the SB transposase. The cartoon depicts integration of the transposon substrate in one blastomere at the two-cell stage. The resulting embryo is predicted to develop as a “half transgenic” animal. b: “Half transgenic” founder. GFP expression in Xenopus laevis founder L2M is restricted to the left side of the dorsal midline (white dashed line). Outcross of this founder, with one-half of the germline containing the transgene, is predicted to generate GFP-positive F1 progeny at a rate of 25%. The observed rate of GFP transmission in the F1 tadpoles was 22% (n = 376/1733).