Fig. 10.

Schematic representation of the predicted integration of a multimeric pT2βGFP transposon concatamer in the X. tropicalis founder line 8F-1. a: Stepwise interplasmid integration and insertion of the concatamer into scaffold 57 of the X. tropicalis genome. It is presumed that multiple, interplasmid incorporation of the transgene occurred prior to integration of the entire concatamer into the genome. Southern blot analysis revealed three GFP-hybridizing bands that were mapped according to size and predicted orientation. b: EPTS LM-PCR products from the left and right SB transposon arms. EPTS product 1 confirms integration into scaffold 57. EPTS product 2 corresponds to vector sequence upstream of the right IR/DR in the SB pT2βGFP transgene. c: Predicted orientation of the multimeric transposon concatamer integration site for X. tropicalis founder 8F. The orientation of transposon 3 (R3 and L3) is predicted from Southern blot data. Schematic map is not to scale. Note: plasmid sequences that reside between transposon elements in the integrated DNA have not been fully characterized and are depicted in the schematic map as gaps between the transposon transgenes.