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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Dev Dyn. 2009 Jul;238(7):1727–1743. doi: 10.1002/dvdy.21994

Fig. 5.

Fig. 5

Verification of the transposon insertion site in founder lines 8F and 9F. a: Genomic PCR products for both the 5’ (right panel) and 3’ (left panel) ends of the integration sites for founder line 8F. Genomic DNA samples were collected from several GFP-positive F1 8F tadpoles (1, 2, 3, 4) and used in PCR reactions to amplify (3’ end 8Fa/8Fb 517 bp fragment; 5’ end 8Fc/8Fd 797 bp). b. Agarose gel electrophoresis of genomic PCR products for founder line 9F. Genomic DNA prepared from three GFP-positive F1 9F tadpoles (1, 2 and 3) was used to amplify the expected sized fragments using primer pairs 9Fa/9Fb (493 bp product) and 9Fc/9Fd (264 bp product). No products were formed when genomic DNA harvested from either a GFP-positive 8F F1 tadpole (8F) or a GFP-negative 9F F1 tadpole (-) was used as the template. L = 100 bp ladder.