Fig. 8.

Schematic of the integration site of the pT2βGFP transgene into scaffold 842 for the X. tropicalis 5M founder line (not to scale). a: Integration of a single transposon transgene integrated into scaffold 842 as determined by Southern blot analysis showing a single ~7 kb BglII digested GFP-positive band. b: Cloning of the flanking genomic sequence by EPTS LM-PCR. EPTS products identified for both the 5’ and 3’ end of the tranposon are noted. c: Final orientation of the transposon integrated into scaffold 842 for X. tropicalis founder line 5M.