Fig. 9.

Map of the integration event in X. tropicalis founder line 9F. The SB pT2βGFP transgene is a concatamer of two transposons integrated into one another followed by integration into the X. tropicalis genome on scaffold 56. a: Interplasmid transposition of transposon one into transposon two followed by integration of the complex into scaffold 56. The map was derived from Southern blot data and shows the predicted orientation of the observed GFP-positive bands (4.5 and 6 kb). b: Flanking sequence was determined by EPTS LM-PCR. Two EPTS restriction fragments digested with NlaIII were identified and correspond to genomic DNA (product 1) and vector sequence (product 2). The 3’ end of the integration site has yet to be determined and is noted by a black box and a question mark. c: Predicted orientation of the transposon concatamer integrated on scaffold 56 within the X. tropicalis founder line 9F. Not to scale.