Table 1.
Sleeping Beauty transgenesis in Xenopus tropicalis and Xenopus laevis.
| Founder ID Number/Gender |
F1 Trangenesis rate # GFP-positive/ total # |
F2 Trangenesis rate # GFP-positive/ total # |
|---|---|---|
| Xenopus tropicalis (SB10 + pT2βGFP) | ||
| Transgenic P0 frogs | ||
| 4M | 48% (n = 185/383) | 49% (n = 479/973) |
| 5M | 6% (n = 13/235) | 51% (n = 258/502) |
| 6M | 1.2% (n = 17/1375) | 49% (n = 710/1437) |
| 7M | 22% (n = 69/314) | 51% (n = 514/1014) |
| 8F | 28% (n= 50/176) | 48% (n= 261/547) |
| 9F | 1.6% (n = 25/1534) | 51% (n = 1459/2878) |
| Non-transgenic P0 frogs | ||
| 1M | 0% (n = 0/172) | NA |
| 2M | 0% (n = 0/259) | NA |
| 3M | 0% (n = 0/1088) | NA |
| 10M | 0% (n = 0/173) | NA |
| 11M | 0% (n = 0/2170) | NA |
| 12M | 0% (n = 0/508) | NA |
| 13F | 0% (n = 0/1802) | NA |
| 14M | 0% (n = 0/1873) | NA |
| 15F | 0% (n = 0/479) | NA |
| Xenopus tropicalis (SB11 + pT2βGFP) | ||
| ♀622E | 56.9% (n = 667/1173) | ND |
| ♂623F | 19.5% (n = 81/415) | ND |
| ♂2262 | 9.7% (n = 273/2825) | ND |
| ♂2232 | 14.5% (n = 372/2562) | ND |
| Xenopus laevis (SB10 + pTβGFP) | ||
| L2M | 22% (n = 376/1733) | ND |
| L3M | 2.9% (n = 35/1211) | ND |
| L4M | 0% (n = 0/541) | NA |
| L5M | 0% (n = 0/46) | NA |
| L6M | 53% (n = 233/441) | ND |
| L7F | 27.5% (n = 22/80) | ND |
NA, not applicable; ND, not done.