Table 2.
Identification of flanking scaffold sequence for several founder lines by EPTS LM-PCR
| Founder Line |
Insertion Site (scaffold:base pair) |
Flanking Scaffold Sequence | Flanking Gene (direction) |
|---|---|---|---|
| Xenopus tropicalis | |||
| 4M | 1092:132184 | TCTGCTGACcttgatc‥ggacattTCCCATTCA | Osteoclast Stimulating Factor 1 (3’) |
| 5M | 842:119502 | TTCTGACATcaatctg‥tatcattAGCCCCAAG | Synaptotagmin- 15 (5’) |
| 8F | 57:2456981 | GCAACGCTagtcacg‥caagttATTGATTA | Kielin (3’) |
| 9F | 56:3221468 |
tagggaTCCCCCCACTGAC (right arm only) |
IGF-II mRNA- binding protein 2 (5’) |
| Xenopus laevis | |||
| L2M | pTβGFP plasmid |
AAAACCCTGACCCagcctct | GFP |
| L2M | pTβGFP plasmid |
CCCCCTTTgggtcaaa | Right arm pTβGFP plasmid |
For the X. tropicalis insertion sites, the scaffold sequence is in uppercase and the lowercase italics shows the transposon plasmid sequence. For the X. laevis data, the breakpoints are indicated in the change from uppercase to lowercase italics font.