Figure 1.
Schematic representations of expression constructs used in this study. (A) p150Glued contains a CAP-Gly microtubule-binding motif near the N terminus and two predicted coiled-coil domains, CC1 (AA 217-548) and CC2 (AA 926-1049). (B) The IC 2C isoform contains a predicted N-terminal coiled-coil domain (AA 1-66), light chain binding regions (RP3, Tctex-1, and LC8: AA 107-138; roadblock: AA 223-261) and WD repeats (AA 262-612). Arrows indicate two sites of alternative splicing (after AA 76 and 106), and the S indicates a region (AA 81-99) that contains eight serine residues. The regions encoded by different expression constructs are shown beneath.