Figure 3.
Velocity sedimentation of detergent lysates from HeLa cells overexpressing dynein IC fragments. Lysates were sedimented into 5-20% sucrose gradients. Fractions were analyzed by Western blotting with antibodies to GFP, dynein IC, the dynein LC, rp3, or the dynactin subunit p150Glued. The construct being expressed and the size of each immunoreactive species are indicated; sedimentation standards are at the bottom. Similar sedimentation patterns were obtained when IC polypeptides were overexpressed in COS-7 cells (our unpublished data).