Table 1.
Effects of dynein IC overexpression on microtubule, centrosome, and endomembrane organization
| Structure scored | GFP | Dynamitin | IC2-FL | N237 | N237 S84A | N237 S84D | N106 |
|---|---|---|---|---|---|---|---|
| Microtubule array | 95.3 | 33.1 | 38.3 | 29.3 | ND | ND | 79.7 |
| γ-Tubulin | 88.8 | 54.0 | 65.3 | 56.0 | ND | ND | 84.3 |
| Centrosomal Arp1 | 84.3 | 39.8 | 45.3 | 36.0 | ND | ND | 72.3 |
| Centrosomal p150 | 93.0 | 19.3 | 28.7 | 26.3 | ND | ND | 77.3 |
| Pericentrin | 93.0 | 92.5 | 94.0 | 93.3 | ND | ND | 94.0 |
| Golgi complex | 97.7 | 8.9 | 84.2 | 23.4 | 22.7 | 55.5 | 90.2 |
| ERGIC | 97.8 | 6.9 | 92.6 | 43.4 | 49.0 | 63.2 | 91.0 |
| Early endosomes | 97.3 | 13.8 | 87.0 | 30.2 | 31.8 | 45.0 | 53.1 |
| Late endosomes | 99.1 | 32.8 | 89.6 | 49.5 | 44.5 | 56.7 | 74.7 |
| Recycling endosomes | 96.8 | 7.4 | 86.4 | 45.2 | 48.9 | 48.3 | 81.5 |
Summary of effects of dynein IC overexpression on subcellular organization. COS-7 cells were used for experiments examining microtubules and centrosomes; HeLa cells were used to evaluate endomembrane compartments. Cells were scored as described in the legends to Figures 4 and 5. The mean percentage of cells showing a normal phenotype is given for each overexpression condition; standard deviations are provided in Figures 4 and 5.